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Developpement d'une application pour identifier en 'real'time' des personnes ayant ete exposes a des radiations ionisantes par une nouvelle technologie de sequencage en 'real time' et portable.

Postée le 06 jan.

Lieu : Oxford · Contrat : Stage · Rémunération : non paye sauf si demande de bourse conjointe peut-etre soumise €

Société : Public Health England

Public Health England (PHE) est une agence executive du department de la sante au Royaune-Uni.un des centres de PHE est iintitule Centre for Radiation, Chemical & Environmental Hazards ou je dirige un laboratoire de recherche (Cancer Mechanisms and Biomarkers group) dan le departement des effets de radiations

Description du poste

Pour un projet de recherche de dosimetrie biologique et d'identification d'individus ayant ete expose aux radiations ionisantes, je souhaite proposer 1 (ou 2) stages d'une duree d'environ 6 mois (a determiner pour mener a bien le projet). Resume du contexte scientifique en anglais ci-dessous

In the event of a large-scale event leading to acute ionizing radiation exposure, high throughput methods would be required to assess individual dose estimates for triage
purposes. Blood-based gene expression is a broad source of biomarkers of radiation exposure which have great potential for providing rapid dose estimates for a large population. Time is a crucial component in radiological emergencies and the shipment of blood samples to relevant laboratories presents a concern. In this study, we performed nanopore sequencing analysis to determine if the technology can be used to detect radiation-inducible genes in human peripheral blood mononuclear cells (PBMCs). The technology offers not only long-read sequencing but also a portable device which can overcome issues involving sample shipment, and provide faster results. The sequencing analysis identified a radiation signature consisting of 46 differentially expressed genes (DEGs) which included 41 protein-coding genes, a long non-coding RNA and four pseudogenes. Extrapolating from the data we obtained, the minimum sequencing time required to detect an irradiated sample using the APOBEC3H transcripts would be less than 3 min for a total of 50,000 reads. Future improvements, in sample processing and informatic pipeline for specific radiation-responsive transcript identification, will allow the
provision of a portable, rapid, real-time biodosimetry platform based on this new sequencing technology.

Profil recherché

Etudiant en fin de Master motive pour un stage en anglais base partiellement a Oxford

Pour postuler :

me contacter directement pour de plus amples informations

christophe.badie@phe.gov.uk